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Image Search Results
Journal: PLoS Pathogens
Article Title: Penton-Dodecahedral Particles Trigger Opening of Intercellular Junctions and Facilitate Viral Spread during Adenovirus Serotype 3 Infection of Epithelial Cells
doi: 10.1371/journal.ppat.1003718
Figure Lengend Snippet: T84 spheroids were infected with mu-Ad3GFP or wt-Ad3GFP at an MOI of 100 vp/cell for 2 hours. Viruses were removed by washing. A ) Spheroids were fixed with 4% PFA at day 4 post-infection. The periphery of spheroids was visualized with rhodamine-labeled Concanavalin A (Con A, red), and cellular nuclei were counterstained with DAPI (blue). Shown are XY and XZ sections. Transduced cells are GFP positive. The scale bars are 50 µm. B ) Culture medium aliquots of the virus-infected T84 spheroids were collected at days 3 and 4 post-infection and titrated on 293 cells. N = 3 **p<0.01.
Article Snippet:
Techniques: Infection, Labeling